pINSPECT:IL2_SigP:NLuc
(Plasmid
#197266)
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PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
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Depositing Lab
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Depositing OrganizationHelmholtz Munich
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197266 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 197266-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 197266-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepINSPECT_SigP:NLuc
- Backbone size w/o insert (bp) 9113
- Total vector size (bp) 13386
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Vector typeMammalian Expression, CRISPR, Luciferase, Synthetic Biology, Unspecified
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Selectable markersPuromycin ; HSV-Thymidine kinase for counter selection using Ganciclovir after removal of selection cassette.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameInterleukin-2 homology arms
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Alt nameIL2 homology arms
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SpeciesH. sapiens (human)
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Insert Size (bp)3720
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 197266-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 197266-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINSPECT:IL2_SigP:NLuc was a gift from Gil Westmeyer (Addgene plasmid # 197266 ; http://n2t.net/addgene:197266 ; RRID:Addgene_197266) -
For your References section:
Intron-encoded cistronic transcripts for minimally invasive monitoring of coding and non-coding RNAs. Truong DJ, Armbrust N, Geilenkeuser J, Lederer EM, Santl TH, Beyer M, Ittermann S, Steinmassl E, Dyka M, Raffl G, Phlairaharn T, Greisle T, Zivanic M, Grosch M, Drukker M, Westmeyer GG. Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 10.1038/s41556-022-00998-6. Epub 2022 Nov 7. 10.1038/s41556-022-00998-6 PubMed 36344775
