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Intron-encoded cistronic transcripts for minimally invasive monitoring of coding and non-coding RNAs.
Truong DJ, Armbrust N, Geilenkeuser J, Lederer EM, Santl TH, Beyer M, Ittermann S, Steinmassl E, Dyka M, Raffl G, Phlairaharn T, Greisle T, Zivanic M, Grosch M, Drukker M, Westmeyer GG
Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 10.1038/s41556-022-00998-6. Epub 2022 Nov 7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
197263pINSPECT_SigP:NLucCloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.
197264pINSPECT_5'-xrRNA_NLuc_3'-XAP1Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
197265pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTRCloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
197266pINSPECT:IL2_SigP:NLucDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
197267pINSPECT:NEAT1-total_SigP:NLucDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
197268pINSPECT:NEAT1-v2_SigP:NLucDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

Antibodies from Article