pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR
(Plasmid #197265)

• Purpose: (Empty Backbone) Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
• Depositing Lab: Gil Westmeyer
• Publication: Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 10.1038/s41556-022-00998-6. Epub 2022 Nov 7.

Backbone

• Vector backbone: pBlueScript-KS II (+)
• Backbone size (bp): 9589
• Vector type: Mammalian Expression, CRISPR, Luciferase, Synthetic Biology, Unspecified
• Selectable markers: Puromycin ; HSV-Thymidine kinase for counter selection using Ganciclovir after removal of selection cassette.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • pINSPECT_5'xrRNA_NLuc_3'HCV-UTR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry