pEGFP-Aurora A
(Plasmid #198402)

• Purpose: Expression of EGFP-tagged Aurora A in mammalian cells
• Depositing Lab: Kenji Sugimoto
• Publication: Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5900
• Modifications to backbone: EcoR I and Sal I sites of pEGFP-ΔC2, which had been derived from pEGFP-C1 by cutting and filling-in Hind III site and converted to C2 reading frame
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Aurora A
• Alt name: BC_001280
• gRNA/shRNA sequence: cDNA
• Species: H. sapiens (human)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Urano, Nagoya Univ.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pEGFP-Aurora-A was constructed by inserting a 1.2 kb fragment of human Aurora-A cDNA into the BglII and SalI site of pEGFP- C1 (Clontech, Palo Alto, CA, USA).