|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||198482||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonelenti-guide puro
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)20
Entrez GeneHgs (a.k.a. H, Hgr, Hrs, ZFYVE8, t, tn)
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer gcctatttcccatgattccttc (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lenti-guide-puro mHgs-2 was a gift from Suzanne Pfeffer (Addgene plasmid # 198482 ; http://n2t.net/addgene:198482 ; RRID:Addgene_198482)
For your References section:Genome-wide screen reveals Rab12 GTPase as a critical activator of pathogenic LRRK2 kinase. Dhekne H.S., Tonelli F., Yeshaw W.M., Chiang C.Y., Limouse C., Jaimon E., Purlyte E., Alessi D.R., Pfeffer S.R.. BioRxiv February 2023 10.1101/2023.02.17.529028