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Lenti-guide-puro mHgs-2
(Plasmid #198482)


Item Catalog # Description Quantity Price (USD)
Plasmid 198482 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    lenti-guide puro
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • gRNA/shRNA sequence
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Hgs (a.k.a. H, Hgr, Hrs, ZFYVE8, t, tn)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer gcctatttcccatgattccttc
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-guide-puro mHgs-2 was a gift from Suzanne Pfeffer (Addgene plasmid # 198482 ; ; RRID:Addgene_198482)
  • For your References section:

    Genome-wide screen reveals Rab12 GTPase as a critical activator of pathogenic LRRK2 kinase. Dhekne H.S., Tonelli F., Yeshaw W.M., Chiang C.Y., Limouse C., Jaimon E., Purlyte E., Alessi D.R., Pfeffer S.R.. BioRxiv February 2023 10.1101/2023.02.17.529028