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pCDNA3-myc3-FBW5 delta F
(Plasmid #19912)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19912 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-myc3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5437
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    F-box and WD repeat domain containing 5 delta F
  • Alt name
    FBW5 delta F
  • Alt name
    FBXW5 delta F
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1752
  • Mutation
    deleted aa1-42 (F-box)
  • GenBank ID
    NM_018998
  • Entrez Gene
    FBXW5 (a.k.a. Fbw5)
  • Tag / Fusion Protein
    • myc3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI/ XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

F-Box Deletion Mutant

(L45M mutation present but does not affect function)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-myc3-FBW5 delta F was a gift from Yue Xiong (Addgene plasmid # 19912 ; http://n2t.net/addgene:19912 ; RRID:Addgene_19912)
  • For your References section:

    WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase. Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y. Genes Dev. 2008 Apr 1. 22(7):866-71. 10.1101/gad.1624008 PubMed 18381890