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pEGFP-PCNA
(Plasmid #199702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199702 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5600
  • Modifications to backbone
    EcoR I and Sal I sites of pEGFP-ΔC2, which had been derived from pEGFP-C1 by cutting and filling-in Hind III site and converted to C2 reading frame
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PCNA
  • Alt name
    NM_182649
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site Bam HI (unknown if destroyed)
  • 5′ sequencing primer EGFP side
  • 3′ sequencing primer SV40 Poly A side
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Origene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

790bp PCR fragment of PCNA (PCNA NM_182649) into PCNA to XhoI-BamHI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-PCNA was a gift from Kenji Sugimoto (Addgene plasmid # 199702 ; http://n2t.net/addgene:199702 ; RRID:Addgene_199702)

  • For your References section:

    Molecular Behavior of CENP-A and PCNA throughout the Cell Cycle in Living Human HT-1080 Cells. Nakagawa C, Senda-Murata K, Kawakita-Yamaguchi A, Fukada T, Sugimoto K. Mol Biol 2016, 5:2. doi: 10.4172/2168-9547.1000160 10.4172/2168-9547.1000160
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