pKrox24(2xD-E)DsRed
(Plasmid #200111)

• Purpose: Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity
• Depositing Lab: Pavel Krejčí
• Publication: Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.

Backbone

• Vector backbone: pDsRed-Express-DR
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: modified EGR1 promoter
• Species: Synthetic
• Mutation: highly modified sequence
• Promoter: 2 copies of D-E element, no other promoter elements

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • 117_Double_pDsRed-Ex-DR(pKrox242xD-EDsRed)[2].gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please Note: Plasmid contains an A to G nucleotide mutation in one of the three D elements of the EGR1D promoter. This mutation is not known to affect plasmid function.