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Addgene

pLV-SV40-puro-EF1a-HA-NLGN1-bc
(Plasmid #200167)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200167 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 10119
  • Total vector size (bp) 12624
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neuroligin-1
  • Alt name
    NLGN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2505
  • GenBank ID
    NM_014932.3
  • Entrez Gene
    NLGN1 (a.k.a. NL1, NLG1)
  • Promoter EF1a
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-SV40-puro-EF1a-HA-NLGN1-bc was a gift from Paul Blainey (Addgene plasmid # 200167 ; http://n2t.net/addgene:200167 ; RRID:Addgene_200167)

  • For your References section:

    High-content image-based pooled screens reveal regulators of synaptogenesis. Le A, Biederer T, Blainey PC. Cell Rep. 2025 Jun 24;44(7):115889. doi: 10.1016/j.celrep.2025.115889. 10.1016/j.celrep.2025.115889 PubMed 40560725
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