|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20021||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2720
Vector typeEntry vector
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsAuthor has listed this plasmid as "medium copy"
Copy numberHigh Copy
Gene/Insert nameTrinucleotide repeat containing 6A
SpeciesH. sapiens (human)
Insert Size (bp)7396
Entrez GeneTNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
- Cloning method Restriction Enzyme
- 5′ cloning site XmnI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR4 TNRC6A was a gift from Thomas Tuschl (Addgene plasmid # 20021 ; http://n2t.net/addgene:20021 ; RRID:Addgene_20021)
For your References section:Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T. RNA. 2008 Oct 31. ():. 10.1261/rna.1351608 PubMed 18978028