pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
(Plasmid
#200277)
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PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200277 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBbE8K
- Backbone size w/o insert (bp) 3487
- Total vector size (bp) 7504
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Modifications to backboneElements for arabinose-inducible expression were replaced with the alanine racemase gene and kanamycin gene flanked with FRT sites
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCadC
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SpeciesEscherichia coli
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Insert Size (bp)1539
Gene/Insert 2
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Gene/Insert nameNanR
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SpeciesEscherichia coli
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Insert Size (bp)792
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP was a gift from Matthew Chang (Addgene plasmid # 200277 ; http://n2t.net/addgene:200277 ; RRID:Addgene_200277) -
For your References section:
Engineering probiotics to inhibit Clostridioides difficile infection by dynamic regulation of intestinal metabolism. Koh E, Hwang IY, Lee HL, De Sotto R, Lee JWJ, Lee YS, March JC, Chang MW. Nat Commun. 2022 Jul 4;13(1):3834. doi: 10.1038/s41467-022-31334-z. 10.1038/s41467-022-31334-z PubMed 35787625
Map uploaded by the depositor.
