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Addgene

pαH-NiV-F-WT-Foldon
(Plasmid #200391)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 200391 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4653
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NiV-F Ectodomain
  • Alt name
    Nipah virus
  • Tag / Fusion Protein
    • Foldon, HRV3C cleavage site, 8X His tag, 2X Strep-Tag II (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from a plasmid made by Jason McLellan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-NiV-F-WT-Foldon was a gift from Priyamvada Acharya (Addgene plasmid # 200391 ; http://n2t.net/addgene:200391 ; RRID:Addgene_200391)

  • For your References section:

    Structures of Langya Virus Fusion Protein Ectodomain in Pre- and Postfusion Conformation. May AJ, Pothula KR, Janowska K, Acharya P. J Virol. 2023 Jun 29;97(6):e0043323. doi: 10.1128/jvi.00433-23. Epub 2023 Jun 6. 10.1128/jvi.00433-23 PubMed 37278642
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