PGK EGFP-LC3B
(Plasmid #200427)

• Purpose: Mammalian expression of fluorescently-tagged marker for autophagic vesicles
• Depositing Lab: Erika Holzbaur
• Publication: Dou et al Cell Rep. 2023 May 1;42(5):112448. doi: 10.1016/j.celrep.2023.112448.

Backbone

• Vector backbone: pEGFPc1/PGK
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAP1LC3B
• Alt name: microtubule associated protein 1 light chain 3 beta
• Alt name: LC3B
• Alt name: ATG8F
• Species: H. sapiens (human)
• Insert Size (bp): 405
• GenBank ID: NM_022818.5
• Promoter: PGK
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: 5'-TAGTGTGGGCCCTGTTCCTG
• 3′ sequencing primer: 5'-CTTTATTTGTAACCATTATAAGCTGC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Modified from PGK mCherry-LC3B

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry