pCA0.325
(Plasmid
#200622)
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Purpose(Empty Backbone) Level 0 RBS acceptor vector with LacZ compatible blue/white screening.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepL0-SC
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Operon building system compatible with the CyanoGate Kit. McCormick Lab also uses TOP10 cells for transformation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCA0.325 was a gift from Alistair McCormick (Addgene plasmid # 200622 ; http://n2t.net/addgene:200622 ; RRID:Addgene_200622) -
For your References section:
CyanOperon: an operon building expansion for the CyanoGate MoClo toolkit. Astbury MJ, Schiavon Osorio AA, Victoria AJ, McCormick AJ. bioRxiv 2026.02.24.707249 10.64898/2026.02.24.707249