pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA
(Plasmid #200641)

• Purpose: Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.
• Depositing Labs: Justin English, Fleur Ferguson
• Publication: Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi: 10.1038/s41467-023-43507-5.

Backbone

• Vector backbone: pCDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6662
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miniTurboID-6xGGSG linker-FKBPF36V-2xHA
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1269
• Promoter: T7
• Tag / Fusion Protein:
  • HA-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pcdna3-1_biotac.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.08.21.554211 for bioRxiv preprint.