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Addgene

RS314-U6(PolII-Lsm)
(Plasmid #200772)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200772 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS314
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 4783
  • Total vector size (bp) 6045
  • Modifications to backbone
    The WT U6 is driven by a GAL1 promoter, U6 coding sequence is inserted between SNR14(-100/-1) and SNR14(+296/+701), in the pRS314 vector
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Gal-SNR6-II
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1262

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CCGCTCGAGATCATATTACATGGCATTACCA
  • 3′ sequencing primer CGGGGTACCTTCCTCTCTGCTGTTTTAG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RS314-U6(PolII-Lsm) was a gift from Aaron Hoskins (Addgene plasmid # 200772 ; http://n2t.net/addgene:200772 ; RRID:Addgene_200772)
  • For your References section:

    Yeast U6 snRNA made by RNA polymerase II is less stable but functional. Lipinski KA, Chi J, Chen X, Hoskins AA, Brow DA. RNA. 2022 Dec;28(12):1606-1620. doi: 10.1261/rna.079328.122. Epub 2022 Oct 4. 10.1261/rna.079328.122 PubMed 36195346