pMM7
(Plasmid #201213)

• Purpose: Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.
• Depositing Lab: Alex Nielsen
• Publication: Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 10.1186/s13568-023-01544-9.

Backbone

• Vector backbone: pMTL61110
• Backbone manufacturer: Sheng et al. 2017
• Total vector size (bp): 6091
• Modifications to backbone: Incorporation of thermostable sfGFP into vector backbone, addition of homologous flanks for targeted deletion of spo0A in the genome of the P. thermoglucosidasius.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 6.25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: If grown in P. thermoglucosidasius: 12.5 mg/L kanamycin, 52 °C
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: sfGFP
• Alt name: Superfolder Green Fluorescent Protein
• Insert Size (bp): 717
• Mutation: F11V, T13P, N39D, A179A

Gene/Insert 2

• Gene/Insert name: Spo0A right flank
• Alt name: P. thermoglucosidasius
• Insert Size (bp): 750

Gene/Insert 3

• Gene/Insert name: Spo0A left flank
• Alt name: P. thermoglucosidasius
• Insert Size (bp): 750

Resource Information

• Supplemental Documents:
  • pMM7-GFP-spo0A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry