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pGFP-mTet1s-delCys pc3904
(Plasmid #201702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201702 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    EGFP-Tet1 (pc2271)
  • Backbone size w/o insert (bp) 6977
  • Total vector size (bp) 10706
  • Modifications to backbone
    The sequences encoding the CRD of GFP-Tet1s (pc3904) were deleted by overlap-extension PCR and the amplicon obtained was used to replace the Tet1 coding sequence in EGFP-Tet1 (pc2271).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tet1s
  • Alt name
    Tet1s Cysteine rich domain deletion
  • Species
    M. musculus (mouse)
  • Mutation
    Deletion of the Cysteine rich domain of Tet1s
  • Entrez Gene
    Tet1 (a.k.a. 2510010B09Rik, Cxxc6, D10Ertd17e, LCX, mKIAA1676)
  • Promoter CAG
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AsiSI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer ATAAGCAGCGATCGCATGGACTGCAGTAGAC
  • 3′ sequencing primer CGTTAACTGCGGCCGCTTAGACCCAAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFP-mTet1s-delCys pc3904 was a gift from Cristina Cardoso (Addgene plasmid # 201702 ; http://n2t.net/addgene:201702 ; RRID:Addgene_201702)

  • For your References section:

    Isoform-specific and ubiquitination dependent recruitment of Tet1 to replicating heterochromatin modulates methylcytosine oxidation. Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, Ludwig AK, Carell T, Cardoso MC. Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8. 10.1038/s41467-022-32799-8 PubMed 36056023
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