lenti.DTR.GFP
(Plasmid #201962)

• Purpose: Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP
• Depositing Lab: Bernhard Schmierer
• Publication: Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi: 10.1038/s41467-023-40688-x.

Backbone

• Vector backbone: lenti.Cas9.BFP.Blast, #196714
• Backbone size w/o insert (bp): 5738
• Total vector size (bp): 7099
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Diptheria Toxin Receptor
• Alt name: DTR, HBEGF
• Species: Chlorocebus sabaeus
• Insert Size (bp): 1362
• GenBank ID: XM_008014669.2
• Promoter: EF1alpha
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsiWI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: tctagaactagtggatc
• 3′ sequencing primer: GATACCGTCGACCTCGA

Resource Information

• Supplemental Documents:
  • 165_Lenti-DTR-EGFP.dna
• A portion of this plasmid was derived from a plasmid made by: Insert was PCR amplified from pAAV-FLEX-DTR-GFP (Addgene #124364, a gift from Eiman Azim & Thomas Jessell)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry