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PurposeMapping of mesenchymal progenitor cells.
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Depositing Lab
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Depositing OrganizationUniversity of Connecticut Health Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 110209-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 110209-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBluescript SKII+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 7700
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Modifications to backboneRestriction sites added and rearranged in the vector MCS.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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Alt nameRFPcherry
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SpeciesDiscosoma species
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Insert Size (bp)711
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GenBank IDAY678264.1
- Promoter Smooth muscle alpha-actin and first intron
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal1 (destroyed during cloning)
- 3′ cloning site BsrG1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymCherry RFP obtained from the lab of Dr. Roger Tsien at UCSD. SMAA promoter+intron fragment obtained from the Fagin & Smith lab at the University of Cincinnati College of Medicine.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 110209-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 110209-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SMA-Cherry was a gift from David Rowe (Addgene plasmid # 110209 ; http://n2t.net/addgene:110209 ; RRID:Addgene_110209) -
For your References section:
In vivo fate mapping identifies mesenchymal progenitor cells. Grcevic D, Pejda S, Matthews BG, Repic D, Wang L, Li H, Kronenberg MS, Jiang X, Maye P, Adams DJ, Rowe DW, Aguila HL, Kalajzic I. Stem Cells. 2012 Feb;30(2):187-96. doi: 10.1002/stem.780. 10.1002/stem.780 PubMed 22083974
Map uploaded by the depositor.
