SMA-Cherry
(Plasmid #110209)

• Purpose: Mapping of mesenchymal progenitor cells.
• Depositing Lab: David Rowe
• Publication: Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi: 10.1002/stem.780.

Backbone

• Vector backbone: pBluescript SKII+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 7700
• Modifications to backbone: Restriction sites added and rearranged in the vector MCS.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Alt name: RFPcherry
• Species: Discosoma species
• Insert Size (bp): 711
• GenBank ID: AY678264.1
• Promoter: Smooth muscle alpha-actin and first intron
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (destroyed during cloning)
• 3′ cloning site: BsrG1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry RFP obtained from the lab of Dr. Roger Tsien at UCSD. SMAA promoter+intron fragment obtained from the Fagin & Smith lab at the University of Cincinnati College of Medicine.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry