DMP1-GFPtopaz
(Plasmid #114270)

• Purpose: GFP labeling of mature osteoblasts and osteocytes.
• Depositing Lab: David Rowe
• Publication: Kalajzic et al Bone. 2004 Jul;35(1):74-82. doi: 10.1016/j.bone.2004.03.006.

Backbone

• Vector backbone: pBluescript SKII+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 16800
• Modifications to backbone: Restriction sites added and rearranged in the vector MCS.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Dmp1 + GFPtopaz
• Species: M. musculus (mouse); Aequorea victoria
• Insert Size (bp): 13800
• GenBank ID: NM001359013 JX472996
• Entrez Gene: Dmp1 (a.k.a. AG1, DMP-1, Dmp, PP)
• Promoter: Dmp1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: T3 or M13R
• 3′ sequencing primer: T7 or M13F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dmp1 promoter + first intron fragment was isolated and cloned into pBS-GFP vector in the lab of Dr. Steve Harris in the Department of Oral Biology, School of Dentistry, University of Missouri-Kansas City. Topaz GFP was purchased from Packard (originally from Aurora).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry