hOC-GFPtpz
(Plasmid
#110207)
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PurposeExpresses GFP in mature bone cells.
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Depositing Lab
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Depositing OrganizationUniversity of Connecticut Health Center
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 110207-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 110207-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC18
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Backbone manufacturerUnknown
- Backbone size w/o insert (bp) 2700
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Modifications to backboneNone
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFPtopaz
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SpeciesAequorea victoria
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Insert Size (bp)720
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GenBank ID
- Promoter Human osteocalcin (BGLAP)
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind3 (unknown if destroyed)
- 3′ cloning site Hind3 (unknown if destroyed)
- 5′ sequencing primer M13forward
- 3′ sequencing primer M13reverse
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byReceived osteocalcin portion of construct from Dr. Edith Gardiner, who was associated with the Garvan Institute in Australia at the time. GFPtopaz was purchased from Packard.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 110207-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 110207-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hOC-GFPtpz was a gift from David Rowe (Addgene plasmid # 110207 ; http://n2t.net/addgene:110207 ; RRID:Addgene_110207) -
For your References section:
Visualizing levels of osteoblast differentiation by a two-color promoter-GFP strategy: Type I collagen-GFPcyan and osteocalcin-GFPtpz. Bilic-Curcic I, Kronenberg M, Jiang X, Bellizzi J, Mina M, Marijanovic I, Gardiner EM, Rowe DW. Genesis. 2005 Oct;43(2):87-98. doi: 10.1002/gene.20156. 10.1002/gene.20156 PubMed 16149065
Map uploaded by the depositor.
