pOBCol2.3-GFPemd
(Plasmid
#110210)
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PurposeLabels cells in the late osteoblast lineage.
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Depositing Lab
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Depositing OrganizationUniversity of Connecticut Health Center
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 110210-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 110210-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC18
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Backbone manufacturerUnknown
- Backbone size w/o insert (bp) 2700
- Total vector size (bp) 7500
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Modifications to backboneHind3 and EcoR1 sites in MCS converted to Sal1 sites with adapters.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFPemerald
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Alt nameEmerald
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SpeciesA. victoria
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Insert Size (bp)720
- Promoter 2.3 kb rat type 1 collagen + first intron
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind3 (destroyed during cloning)
- 3′ cloning site EcoR1 (destroyed during cloning)
- 5′ sequencing primer M13reverse
- 3′ sequencing primer M13forward
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFPemerald purchased from Packard.
DNA (Catalog # 110210-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 110210-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOBCol2.3-GFPemd was a gift from David Rowe (Addgene plasmid # 110210 ; http://n2t.net/addgene:110210 ; RRID:Addgene_110210) -
For your References section:
Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler AC, Rowe D. J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 10.1359/jbmr.2002.17.1.15. 10.1359/jbmr.2002.17.1.15 PubMed 11771662
Map uploaded by the depositor.
