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pRBUBR1
(Plasmid #20198)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20198 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Yeplac 181
  • Backbone size w/o insert (bp) 5741
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBR1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    UBR1 (a.k.a. YGR184C, PTR1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer yADH1-F
  • 3′ sequencing primer M13-puc-Rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid for overexpressing Ubr1ha in yeast. The ha tag is at the C-terminus of the protein and the protein is active in degrading N-end rule substrates.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRBUBR1 was a gift from Alexander Varshavsky (Addgene plasmid # 20198 ; http://n2t.net/addgene:20198 ; RRID:Addgene_20198)
  • For your References section:

    Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway. Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A. J Biol Chem. 2008 Aug 29. 283(35):24011-28. 10.1074/jbc.M802583200 PubMed 18566452