pRBUBR1
(Plasmid #20198)

• Depositing Lab: Alexander Varshavsky
• Publication: Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-28.

Backbone

• Vector backbone: Yeplac 181
• Backbone size w/o insert (bp): 5741
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBR1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: UBR1 (a.k.a. YGR184C, PTR1)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: yADH1-F
• 3′ sequencing primer: M13-puc-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid for overexpressing Ubr1ha in yeast. The ha tag is at the C-terminus of the protein and the protein is active in degrading N-end rule substrates.