Px-PhiYFP-triplex-DR-M13-DR-pA
(Plasmid
#202046)
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PurposeEncodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, dCas12a DRs, and cloning site for crRNA to activate downstream targets.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepGL2-Luc
- Total vector size (bp) 4279
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePhiYFP
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gRNA/shRNA sequenceM13
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.05.08.539911 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Px-PhiYFP-triplex-DR-M13-DR-pA was a gift from Darren Cusanovich (Addgene plasmid # 202046 ; http://n2t.net/addgene:202046 ; RRID:Addgene_202046) -
For your References section:
Optimization of a Cas12a-Driven Synthetic Gene Regulatory Network System. Kang H, Fitch JC, Varghese RP, Thorne CA, Cusanovich DA. ACS Synth Biol. 2025 May 16;14(5):1732-1744. doi: 10.1021/acssynbio.5c00084. Epub 2025 May 2. 10.1021/acssynbio.5c00084 PubMed 40316310