Skip to main content
Addgene

Px-mCherry-pA
(Plasmid #202049)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202049 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pGL2-Luc
  • Total vector size (bp) 3871
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct contains a cloning site for building a synthetic promoter that can be targeted by Cas transactivators. The plasmid is provided with BsmBI sites and M13 sequence that can be cut out and replaced with the desired target sequence. Please see the attached protocol for details on building synthetic Cas promoters. Please visit https://doi.org/10.1101/2023.05.08.539911 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Px-mCherry-pA was a gift from Darren Cusanovich (Addgene plasmid # 202049 ; http://n2t.net/addgene:202049 ; RRID:Addgene_202049)

  • For your References section:

    Optimization of a Cas12a-Driven Synthetic Gene Regulatory Network System. Kang H, Fitch JC, Varghese RP, Thorne CA, Cusanovich DA. ACS Synth Biol. 2025 May 16;14(5):1732-1744. doi: 10.1021/acssynbio.5c00084. Epub 2025 May 2. 10.1021/acssynbio.5c00084 PubMed 40316310
Related items: