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Ub[9]-mRFP[1]
(Plasmid #20249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20249 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 20249-D50 50 μg of DNA in Tris buffer $495
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DNA 20249-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    mRFP-N1
  • Backbone size w/o insert (bp) 4678
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin C
  • Alt name
    UBC
  • Alt name
    UBC-mRFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6755
  • Mutation
    changed Stop codon 686 to Glycine
  • GenBank ID
    NM_021009
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Tag / Fusion Protein
    • mRFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ub[9]-mRFP[1] was a gift from Nico Dantuma (Addgene plasmid # 20249 ; http://n2t.net/addgene:20249 ; RRID:Addgene_20249)

  • For your References section:

    Selective accumulation of aggregation-prone proteasome substrates in response to proteotoxic stress. Salomons FA, Menendez-Benito V, Bottcher C, McCray BA, Taylor JP, Dantuma NP. Mol Cell Biol. 2009 Apr;29(7):1774-85. doi: 10.1128/MCB.01485-08. Epub 2009 Jan 21. 10.1128/MCB.01485-08 PubMed 19158272
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