pOTTC2147 - pAAV nEF ConFon hChR2(H134R)-mCherry
(Plasmid #202545)

• Purpose: An INTRSECT-ready adeno-associated viral vector expressing channelrhodopsin2-mCherry fusion (Cre-On and Flp-On)
• Depositing Labs: Marisela Morales, Christopher Richie
• Publication: Barbano et al Nat Commun. 2025 Sep 26;16(1):8500. doi: 10.1038/s41467-025-63396-0.

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Backbone

• Vector backbone: pAAV-nEF Con/Fon hChR2(H134R)-EYFP
• Backbone manufacturer: Karl Deisseroth (Addgene plasmid # 55644)
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hChR2(H134R)-mCherry
• Species: Synthetic
• Insert Size (bp): 2106
• Promoter: nEF

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • pOTTC2147 - pAAV nEF ConFon hChR2(H134R)-mCherry .gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry