pETM11-6xHis-TEV-hORF1p
(Plasmid
#202589)
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PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETM11
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 6369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameORF1p
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Alt nameORF1
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Alt nameL1ORF1p
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Alt namep40
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SpeciesH. sapiens (human)
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Insert Size (bp)1090
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Tags
/ Fusion Proteins
- 6x His (N terminal on insert)
- TEV protease cleavage site (N terminal on insert)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was originally constructed by Martin Taylor in the lab of Kathleen Burns.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETM11-6xHis-TEV-hORF1p was a gift from Kathleen Burns & Liam Holt (Addgene plasmid # 202589 ; http://n2t.net/addgene:202589 ; RRID:Addgene_202589) -
For your References section:
Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition. Taylor MS, LaCava J, Mita P, Molloy KR, Huang CR, Li D, Adney EM, Jiang H, Burns KH, Chait BT, Rout MP, Boeke JD, Dai L. Cell. 2013 Nov 21;155(5):1034-48. doi: 10.1016/j.cell.2013.10.021. 10.1016/j.cell.2013.10.021 PubMed 24267889