psiCHECK2 RPS28 UTR1 WT
(Plasmid #203157)

• Purpose: RPS28 UTR luciferase assay plasmid (WT UTR1, sites 1 and 2)
• Depositing Lab: Susan Baserga
• Publication: Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):1988-2011. doi: 10.1093/nar/gkad1235.

Backbone

• Vector backbone: psiCHECK2
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPS28 UTR
• Species: H. sapiens (human)
• Insert Size (bp): 207
• Entrez Gene: RPS28 (a.k.a. DBA15, S28, eS28)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: TCCAGAGCCTGTCCACGTAT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

207 bp region of the human RPS28 UTR containing two MIR-28 binding sites (WT). Cloned from MCF10A cell line genomic DNA.

Please visit https://pubmed.ncbi.nlm.nih.gov/36824951/ for bioRxiv preprint.