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Addgene

Vps4p_GP_linker
(Plasmid #203361)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203361 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET151
  • Backbone size w/o insert (bp) 5751
  • Total vector size (bp) 7065
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vps4
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1314
  • Mutation
    Residues 83-111 are replaced with Gly Pro linker
  • Promoter LacO
  • Tag / Fusion Protein
    • 6x-HIS-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Vps4p_GP_linker was a gift from Chris Hill (Addgene plasmid # 203361 ; http://n2t.net/addgene:203361 ; RRID:Addgene_203361)

  • For your References section:

    Substrate binding and coupled mechanisms of Vps4p substrate recruitment and release from autoinhibition. Wienkers H, Han H, Whitby F, Hill CP. Sci Rep. 2025 Jul 11;15(1):25024. doi: 10.1038/s41598-025-08418-z. 10.1038/s41598-025-08418-z PubMed 40646008
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