pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald
(Plasmid #203798)

• Purpose: Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
• Depositing Labs: Richard Eva, Bart Nieuwenhuis
• Publication: Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. doi: 10.15252/emmm.201911674. Epub 2020 Jun 17.

Backbone

• Vector backbone: pHRsinUbEm
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PIK3CA
• Alt name: Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha
• Alt name: P110alpha
• Species: H. sapiens (human)
• Insert Size (bp): 3207
• Mutation: H1047R
• Entrez Gene: PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)

Gene/Insert 2

• Gene/Insert name: Emerald
• Alt name: EmGFP
• Species: A. victoria
• Insert Size (bp): 717

Resource Information

• Supplemental Documents:
  • pLV-SFFV-PIK3CA H1047R-UbC-Emerald.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry