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pUN1308 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pNMT
(Plasmid #203900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB90
  • Backbone manufacturer
    Mortensen et al., 2019 (doi:10.3389/fpls.2019.00755)
  • Vector type
    Plant Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pNMT-FLUC-tOCS; pNOS-RLUC-tNOS
  • Species
    Synthetic; Catharanthus roseus var. LBE
  • Insert Size (bp)
    5283

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUN1308 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pNMT was a gift from Carolyn Lee-Parsons (Addgene plasmid # 203900 ; http://n2t.net/addgene:203900 ; RRID:Addgene_203900)

  • For your References section:

    Identification of DELLA and GID1 genes in Catharanthus roseus and their potential role in regulating vindoline biosynthesis. Cole-Osborn LF, Soens N, Bernal-Franco D, Prifti O, Cram EJ, Lee-Parsons CWT. Plant Mol Biol. 2025 Jun 5;115(3):72. doi: 10.1007/s11103-025-01599-1. 10.1007/s11103-025-01599-1 PubMed 40471459
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