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Addgene

pJBL830
(Plasmid #204136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204136 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p15A oripACYC
  • Total vector size (bp) 3208
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    T. pe fluoride riboswitch with decreasing mutant at position 4 regulated sfGFP
  • Alt name
    superfolder Green Fluorescent Protein
  • Species
    Synthetic
  • Insert Size (bp)
    714

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBL830 was a gift from Julius Lucks (Addgene plasmid # 204136 ; http://n2t.net/addgene:204136 ; RRID:Addgene_204136)

  • For your References section:

    The effect of pseudoknot base pairing on cotranscriptional structural switching of the fluoride riboswitch. Hertz LM, White EN, Kuznedelov K, Cheng L, Yu AM, Kakkaramadam R, Severinov K, Chen A, Lucks JB. Nucleic Acids Res. 2024 May 8;52(8):4466-4482. doi: 10.1093/nar/gkae231. 10.1093/nar/gkae231 PubMed 38567721
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