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anti-Calbindin scFv [L109/57] with sortase tag
(Plasmid #204421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204421 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.4
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 6011
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Recombinant mouse scFv targeting Calbindin (Homo sapiens)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    849
  • Tags / Fusion Proteins
    • Sortase tag (C terminal on insert)
    • 6xHis tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.05.20.540091 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    anti-Calbindin scFv [L109/57] with sortase tag was a gift from Jeff Lichtman (Addgene plasmid # 204421 ; http://n2t.net/addgene:204421 ; RRID:Addgene_204421)

  • For your References section:

    Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex. Han X, Lu X, Li PH, Wang S, Schalek R, Meirovitch Y, Lin Z, Adhinarta J, Murray KD, MacNiven LM, Berger DR, Wu Y, Fang T, Meral ES, Asraf S, Ploegh H, Pfister H, Wei D, Jain V, Trimmer JS, Lichtman JW. Nat Commun. 2024 Aug 5;15(1):6648. doi: 10.1038/s41467-024-50411-z. 10.1038/s41467-024-50411-z PubMed 39103318
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