pV1F
(Plasmid #205084)

• Purpose: Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.
• Depositing Lab: Suomeng Dong
• Publication: Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346. doi: 10.1371/journal.ppat.1011346. eCollection 2023 Apr.

Backbone

• Vector backbone: p302T
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AtVIP1
• Alt name: arabidopsis VirE2-interacting Protein 1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1023
• GenBank ID: NC_003070.9
• Promoter: agorbacterium virB operon promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: CGGGGTACCATGCCTGTCGAGTCGGCTGAGAATATA
• 3′ sequencing primer: CCGGAATTCTCATAGACCGCGCGTTGATC

Resource Information

• Supplemental Documents:
  • pV1F-KanR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry