pInt_attP1_kanR
(Plasmid
#205294)
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Purpose(Empty Backbone) Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205294 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepKD4
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BW25141
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypKM496 (Addgene #109301) and pKD4 (CGSC #7632)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Nonreplicating ORBIT integrating plasmid with WT attP (P1) sequence. Replicates in pir+ strains for cloning. FRT sites can be used to excise most of the backbone. Kanamycin resistant. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Depositing lab uses BW25141 strain for growth (https://cgsc.biology.yale.edu/Strain.php?ID=64690).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pInt_attP1_kanR was a gift from Scott Saunders (Addgene plasmid # 205294 ; http://n2t.net/addgene:205294 ; RRID:Addgene_205294) -
For your References section:
ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency. Saunders SH, Ahmed AM. bioRxiv 2023 10.1101/2023.06.28.546561
Map uploaded by the depositor.
