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(Plasmid #214467)


Item Catalog # Description Quantity Price (USD)
Plasmid 214467 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Plasmid has temperature sensitive replication and is lost at 37°C or 42°C. It must be grown at 30°C to replicate.
  • Copy number
    Low Copy


  • Gene/Insert name
    CspRecT / Bxb-1
  • Species
  • Promoter XylS / AraC

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    George Church (Addgene plasmid # 138474: pORTMAGE-Ec1), Kenan Murphy (Addgene plasmid # 108320: pKM461), and Coli Genetic Stock Center (CGSC #7739: pKD46).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHelper_TS_V2_ampR was a gift from Scott Saunders (Addgene plasmid # 214467 ; ; RRID:Addgene_214467)
  • For your References section:

    ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency. Saunders SH, Ahmed AM. bioRxiv 2023 10.1101/2023.06.28.546561