ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency

Saunders SH, Ahmed AM
bioRxiv 2023 Article

Plasmids from Article

ID	Plasmid	Purpose
205291	pHelper_Ec1_V1_gentR	Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
205292	pHelper_Ec1_V1_ampR	Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
205293	pHelper_NoMutL_V2_gentR	Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
205294	pInt_attP1_kanR	Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
205295	pInt_attP1_chlorR	Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
205296	pInt_attP1_ampR	Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
205297	pInt_attP2_kanR	Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#2).
205298	pInt_attP3_chlorR	Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#3).
205299	pInt_attP5_ampR	Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#5).
205300	pInt_attP1_LCS_kanR	Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.
205301	pInt_attP1_sacB_kanR	Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.
205302	pInt_attP1_tsXis_sacB_kanR	Integrating plasmid for ORBIT in E. coli with self excising factor (Xis) and sacB for counter selection. Used for markerless modifications.
214467	pHelper_TS_V2_ampR	Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.