pInt_attP5_ampR
(Plasmid
#205299)
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Purpose(Empty Backbone) Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#5).
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205299 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepKD4
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BW25141
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypKM496 (Addgene #109301), pKD4 (CGSC #7632), and pKD46 (CGSC #7739)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Same as pInt_attP1_ampR, except this plasmid has the orthogonal attP5 sequence. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Depositing lab uses BW25141 strain for growth (https://cgsc.biology.yale.edu/Strain.php?ID=64690).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pInt_attP5_ampR was a gift from Scott Saunders (Addgene plasmid # 205299 ; http://n2t.net/addgene:205299 ; RRID:Addgene_205299) -
For your References section:
ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency. Saunders SH, Ahmed AM. bioRxiv 2023 10.1101/2023.06.28.546561
Map uploaded by the depositor.
