TOPO-LG4_chr5:550kb antisense
(Plasmid
#205468)
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PurposeEnhancer containing plasmid used for assessing enhancer::promoter interactions
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205468 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1-Topo
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3900
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Vector typeSynthetic Biology ; subcloning of Taq-amplified PCR products
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namechr5:550kb LG4
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SpeciesH. sapiens (human)
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Insert Size (bp)4550
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer m13 reverse
- 3′ sequencing primer m13 forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TOPO-LG4_chr5:550kb antisense was a gift from Glen Borchert (Addgene plasmid # 205468 ; http://n2t.net/addgene:205468 ; RRID:Addgene_205468) -
For your References section:
Long G4-rich enhancers target promoters via a G4 DNA-based mechanism. DeMeis JD, Roberts JT, Delcher HA, Godang NL, Coley AB, Brown CL, Shaw MH, Naaz S, Dahal A, Alqudah SY, Nguyen KN, Nguyen AD, Paudel SS, Shell JE, Patil SS, Dang H, O'Neal WK, Knowles MR, Houserova D, Gillespie MN, Borchert GM. Nucleic Acids Res. 2025 Jan 11;53(2):gkae1180. doi: 10.1093/nar/gkae1180. 10.1093/nar/gkae1180 PubMed 39658038