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Addgene

psiCHECK-2 GATD1 Alu_Mut1
(Plasmid #205470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205470 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6273
  • Vector type
    RNAi, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mutant 1 GATD1 Alu Domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    78
  • Mutation
    Mutated the entire structure of the alu domain with the addition of ACACAC repeated
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NOTI (not destroyed)
  • 5′ sequencing primer TGGGTAAGTACATCAAG
  • 3′ sequencing primer CTCATTTAGATCCTCAC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psiCHECK-2 GATD1 Alu_Mut1 was a gift from Glen Borchert (Addgene plasmid # 205470 ; http://n2t.net/addgene:205470 ; RRID:Addgene_205470)
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