pEF1-Pls4-sfGFP
(Plasmid
#205751)
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Purpose(Empty Backbone) Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepTRKH2
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Modifications to backboneInsertion of Pls4 inducible cassette
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Vector typeBacterial Expression ; shuttle vector gram+ gram-
- Promoter Pls4 (Phyperspank mutant, IPTG inducible)
Growth in Bacteria
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Bacterial Resistance(s)Erythromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF1-Pls4-sfGFP was a gift from Jerome Bonnet (Addgene plasmid # 205751 ; http://n2t.net/addgene:205751 ; RRID:Addgene_205751) -
For your References section:
LactoSpanks: A Collection of IPTG Inducible Promoters for the Commensal Lactic Acid Bacteria Lactobacillus gasseri. Fristot E, Cambray G, Bonnet J. ACS Synth Biol. 2024 Mar 15;13(3):951-957. doi: 10.1021/acssynbio.3c00438. Epub 2024 Feb 9. 10.1021/acssynbio.3c00438 PubMed 38335132
Map uploaded by the depositor.
