Cav2.1 EA2, R57A, R59A pMT2
(Plasmid
#206122)
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Purposeexpression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents
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Depositing Lab
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Depositing OrganizationUniversity College London
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 206122-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 206122-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMT2
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Backbone manufacturerGenetics Institute Inc
- Backbone size w/o insert (bp) 5163
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecacna1a
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Alt nameCav2.1 alpha1A/EA2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)3792
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MutationCav2.1-P1217fs, R57A, R59A
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Entrez GeneCacna1a (a.k.a. BccA1, Cav2.1, rbA-1)
- Promoter Ad MLP/TPL/SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer AGCTTGAGGTGTGGCAGGCTT
- 3′ sequencing primer GGTCGAACCATGATGGCAGC
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTerry P. Snutch
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 206122-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 206122-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cav2.1 EA2, R57A, R59A pMT2 was a gift from Annette Dolphin (Addgene plasmid # 206122 ; http://n2t.net/addgene:206122 ; RRID:Addgene_206122) -
For your References section:
A CaV2.1 N-terminal fragment relieves the dominant-negative inhibition by an Episodic ataxia 2 mutant. Dahimene S, Page KM, Nieto-Rostro M, Pratt WS, D'Arco M, Dolphin AC. Neurobiol Dis. 2016 Sep;93:243-56. doi: 10.1016/j.nbd.2016.05.020. Epub 2016 May 31. 10.1016/j.nbd.2016.05.020 PubMed 27260834
