PGK EGFP-RAB3A-WT
(Plasmid
#206146)
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PurposePGK EGFP-RAB3A-WT
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePGK pEGFP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3500
- Total vector size (bp) 5400
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Modifications to backboneReplaced CMV with PGK
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAB3A
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SpeciesH. sapiens (human)
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Insert Size (bp)1897
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Entrez GeneRAB3A
- Promoter PGK
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site DraIII (unknown if destroyed)
- 5′ sequencing primer 5'-GACCACTACCAGCAGAACAC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene# 49542
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PGK EGFP-RAB3A-WT was a gift from Erika Holzbaur (Addgene plasmid # 206146)