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pET15-VraS
(Plasmid #206332)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206332 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VraS
  • Species
    Staphylococcus aureus
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (unknown if destroyed)
  • 3′ cloning site Xho I (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15-VraS was a gift from May Abdelaziz (Addgene plasmid # 206332 ; http://n2t.net/addgene:206332 ; RRID:Addgene_206332)

  • For your References section:

    A mechanistic understanding of the effect of Staphylococcus aureus VraS histidine kinase single-point mutation on antibiotic resistance. Ali L, Karki S, Boorgula GD, Mekakda A, Cagle-White B, Bhattarai S, Beaudoin R, Blakeney A, Singh S, Srivastava S, Abdel Aziz MH. Microbiol Spectr. 2025 Apr 15;13(5):e0009525. doi: 10.1128/spectrum.00095-25. 10.1128/spectrum.00095-25 PubMed 40233945
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