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pET15-VraS - A314T
(Plasmid #206333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206333 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VraS
  • Species
    Staphylococcus aureus
  • Mutation
    A314T
  • Tag / Fusion Protein
    • 6x His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (unknown if destroyed)
  • 3′ cloning site Xho I (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15-VraS - A314T was a gift from May Abdelaziz (Addgene plasmid # 206333 ; http://n2t.net/addgene:206333 ; RRID:Addgene_206333)

  • For your References section:

    Staphylococcus aureus sensor histidine kinase VraS mutations linked to antibiotic resistance affect enzymatic catalysis, stability, and substrate interactions. Cagle-White B, Bhattarai S, Sunderhaus A, Scott E, Solis A, Marsh L, Catague KL, Sarkar A, Abdel Aziz MH. Arch Biochem Biophys. 2026 Feb;776:110715. doi: 10.1016/j.abb.2025.110715. Epub 2025 Dec 24. 10.1016/j.abb.2025.110715 PubMed 41453699
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