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Addgene

pJethlpAmScarletSpc
(Plasmid #206821)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206821 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ClonepJetPCR
  • Backbone manufacturer
    Thermo Scientific
  • Backbone size w/o insert (bp) 2715
  • Vector type
    Synthetic Biology
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hlpA
  • Species
    Streptococcus pneumoniae
  • Insert Size (bp)
    2098
  • Tag / Fusion Protein
    • mScarlet (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer M13-R(-46)
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Jan-Willem Veening Département de microbiologie fondamentale (DMF), Université de Lausanne

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ANT(9) gene under its own promoter is placed downstream of hlpA-mScarlet-I gene ; Please visit https://doi.org/10.1101/2025.05.10.653270 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJethlpAmScarletSpc was a gift from Virginie Libante (Addgene plasmid # 206821 ; http://n2t.net/addgene:206821 ; RRID:Addgene_206821)

  • For your References section:

    A Modular Genetic Toolbox for Precise Gene Regulation and Multi-Color Imaging in Streptococci. Mignolet J, Staub J, van der Meer JR, Veening J-W, Libante V. bioRxiv 2025.05.10.653270 10.1101/2025.05.10.653270
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