Skip to main content
Addgene
Open Menu

px-458-sgRNA-IF1
(Plasmid #206923)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206923 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    px458
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 9289
  • Total vector size (bp) 9292
  • Vector type
    CRISPR
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    In LB medium
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting IF1 (ATPase inhibitory factor 1)
  • gRNA/shRNA sequence
    gcagtccgagaatgtcgaccg
  • Species
    H. sapiens (human)
  • GenBank ID
    NC_000001.11
  • Entrez Gene
    ATP5IF1 (a.k.a. ATPI, ATPIF1, ATPIP, IP)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer GACTATCATATGCTTACCGT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

gRNA sequence (gcagtccgagaatgtcgaccg) differs from that listed in the original publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px-458-sgRNA-IF1 was a gift from José Cuezva (Addgene plasmid # 206923 ; http://n2t.net/addgene:206923 ; RRID:Addgene_206923)

  • For your References section:

    IF1 promotes oligomeric assemblies of sluggish ATP synthase and outlines the heterogeneity of the mitochondrial membrane potential. Romero-Carraminana I, Esparza-Molto PB, Dominguez-Zorita S, Nuevo-Tapioles C, Cuezva JM. Commun Biol. 2023 Aug 12;6(1):836. doi: 10.1038/s42003-023-05214-1. 10.1038/s42003-023-05214-1 PubMed 37573449
Related items: