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pIF1-S39E-GFP
(Plasmid #206937)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206937 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP_C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5093
  • Total vector size (bp) 6093
  • Vector type
    Mammalian Expression
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grown in Luria-Bertani medium
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    S39E mutant of human IF1 (ATPase inhibitory factor 1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    Changed serine 39 for a glutamic acid
  • GenBank ID
    NC_000001.11
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer CGTCGCCGTCCAGCTCGACCAG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIF1-S39E-GFP was a gift from José Cuezva (Addgene plasmid # 206937 ; http://n2t.net/addgene:206937 ; RRID:Addgene_206937)

  • For your References section:

    IF1 promotes oligomeric assemblies of sluggish ATP synthase and outlines the heterogeneity of the mitochondrial membrane potential. Romero-Carraminana I, Esparza-Molto PB, Dominguez-Zorita S, Nuevo-Tapioles C, Cuezva JM. Commun Biol. 2023 Aug 12;6(1):836. doi: 10.1038/s42003-023-05214-1. 10.1038/s42003-023-05214-1 PubMed 37573449
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